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Banu, Fouzia
- Silver Nanoparticles from Switenia mahagony: green Synthesis and Characterisation
Authors
1 Department of Biomedical Sciences, Asmara College of Health Sciences, Asmara, Eritrea, North East Africa. P.O.Box 1866
2 Department of Biochemistry, JBAS College for Women, Chennai, IN
3 Department of PG Biochemistry, Bharathi Women’s College North Madras, Chennai., IN
4 Department of Basic and behavioural Sciences, Asmara College of Health Sciences, Asmara, Eritrea, North East Africa. P.O.Box 1866.
Source
Indian Journal of NanoScience, Vol 1, No 1 (2013), Pagination: 1-5Abstract
The synthesis of metal and semiconductor nanoparticles is an expanding research area due to the potential applications for the development of novel technologies. In this work, we describe a cost effective and environment friendly technique for green synthesis of silver phyto nanoparticles and their antibiogram from 3mM silver nitrate solution through the extract of Switenia mahagony as reducing as well as capping agent. In the process of synthesising silver nanoparticles, we observed a rapid reduction of silver ions leading to the formation of stable crystalline silver nanoparticles in the solution. The herbal leaves and their medicinal properties were already discussed in varieties of ayurvedic studies. The synthesis of silver phyto nanoparticles were prepared by adding silver nitrate solution solution (3mM) to the plant extract. Nanoparticles were characterised using UV-Visible absorption spectroscopy, FTIR, XRD, XRF, TEM AND SEM analysis. The biomass of plants produces their nanomaterials by a process called bio mineralisation. It was concluded from the above studies that 3 mM concentration of silver nanoparticles showed the best concentration amongst the various concentrations prepared and also their characterisation showed that these nanoparticles synthesised were mostly globular in structure, and proved to have the functional groups in the terminal ends as well as showed sharp peaks by XRD studies.Keywords
Herbal Extract, Silver Nanoparticles, UV- VIS, FTIR, TEM, SEM, XRDReferences
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- Green Synthesis, and Antimicrobial Activity of Silver Nanoparticles from the Medicinal Plant Vernonia amygdalina
Authors
1 Dhanapalan College of Arts and Science, Kelambakkam, Chennai-603103, IN
2 JBAS College for Women, Teynampet, Chennai-600018, IN
3 Dept of Applied Sciences, PNG, University of Technology, Papua New Guinea, PG
Source
Indian Journal of NanoScience, Vol 1, No 1 (2013), Pagination: 32-35Abstract
Silver nanoparticles of different concentrations were synthesized from the medicinal plant Vernonia amygdalina biologically, as this technique is cost effective and environment friendly. The characterization of the silver nanoparticles was analysed by the UV-Vis Spectrophotometer, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy, X-Ray Diffraction (XRD), and Fourier Transform Infra Red (FTIR). The absorption spectra of silver nanoparticles studied using the UV-Vis spectroscopy, had an absorbance peak at 475nm, in all the three different concentrations (1mM, 3mM and 5mM) of silver nanoparticles. The silver nanoparticles have a 50-70nm size range and appeared to be dominantly spherical and were occasionally triangular. The XRD pattern revealed that these silver nanoparticles have a crystalline nature. The antimicrobial activity of these nanoparticles was studied against E.coli, S.aureus, P.aeuruginosa, and C.albicans. They appeared to have satisfactory inhibitions against the four mentioned microorganisms. Among the different concentrations used in the study, the 3mM (20mg/500μl distilled water) appeared to have the highest sensitivity.Keywords
Amygdalina Vernonia, Bioreduction, Silver Nanoparticles, MicroorganismsReferences
- Vyom Parashar, Rashmi Parashar, Bechan Sharma, Avinash C.Pandey, Digest Journal of [2009]. Nanomaterials and Biostructures, 4(1),45 -50
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- Upendra Kumar Parashar,Preeti S.Saxena,Anchal Srivastava, Digest Journal of Nanomaterials and Biostruct ures,4(1),159-166[2009].
- D.S. Goodsell Bionanotechnology: Lessons from Nature. John Wiley & Sons Inc.Publication (2004).
- E.Mayes, A.Bewick,D Gleeson,J Hoinville,R Jones,IEEE Transactions on Magnetics 39(2), 624(2003).
- Belly, R. T., and G. C. Kydd. 1982. Silver resistance in microorganisms. Dev. Ind. Microbiol. 23:567-577.
- Joseph Wang.,Analytica Chimica Acta 500, 247(2003).
- Antioxidant Activity of Geraniol on n-Nitrosodiethylamine-induced Hepatocarcinogenesis in Wistar Albino Rats
Authors
1 Department of Biochemistry, JBAS College for Women, Chennai, IN
2 Prof. Dhanapalan College of Arts and Science, Chennai, IN
3 PNG University of Technology, IN
4 CLRI, Chennai, IN
Source
Indian Journal of Oncology and Radiation Biology, Vol 2, No 7 (2014), Pagination: 4-9Abstract
The chemopreventive effect of Geraniol was evaluated in N-nitrosodiethylamine-induced experimental liver tumor in Wistar albino rats. In this study the animals were divided into four groups with 6 rats each and received treatments for 4 weeks. Group I served as control (untreated), Group II treated with N-nitrosodiethylamine (DEN) (200 mgkg, i.p.) and Phenobarbital (PB) (0.05%) in drinking water; Group III received Geraniol (250 mg/kg b.wt) in addition to DEN and Group IV treated with Geraniol only for four weeks. The administration of Geraniol in rats with DEN induced HCC rats (Group III) shows a significantly reduced level of liver serum marker (aspartate aminotransferase, alanine aminotransferase, acid phosphatase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transpeptidase, alpha fetoprotein and 5'nucleotidase). These results are evident that Geraniol possesses protective effect against N-Nitrosodiethylamine (DEN)-induced Hepatocellular Carcinogenesis (HCC).Keywords
DEN, Carcinogenesis, HCC, Geraniol, Marker Enzymes- Antimicrobial Activity of Silver Nanoparticles from Pithecellobium Dulce
Authors
1 Deptartment of Biochemistry, Prof. Dhanapalan College, Chennai, IN
2 PNG University of Technology, PNG, IN
3 Department of Biochemistry, JBAS College, Chennai, IN
4 CLRI, Chennai, IN
Source
Indian Journal of NanoScience, Vol 2, No 7 (2014), Pagination: 1-3Abstract
In this present study, the silver nanoparticles synthesized biologically from the medicinal plant Pithecellobium dulce were tested for its antimicrobial activity against E.coli, S.aureus, P.aeuruginosa, and C.albicans. The biologically synthesized nanoparticles exhibited satisfactory inhibitions against all tested microorganisms when used at different concentrations viz. 1 mM, 3 mM and 5 mM; 3 mM (20 mg/500 μl distilled water) offered the highest sensitivity. Such green-synthesized antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue.Keywords
E.coli, C.albicans, P.aeuruginosa, Pithecellobium Dulce, S.aureus- Antimicrobial Activity of Aegle Marmelos (correa) Linn. Silver Nanoparticles
Authors
1 Deptartment of Medical Biochemistry, University of Madras, Chennai, IN
2 Department of Biochemistry JBAS College, Chennai, IN
3 CLRI, Chennai, IN
4 PNG University of Technology, PG
5 Department of Plant Biology and plant Biotechnology, Presidency College, Chennai-600 005, IN
Source
Indian Journal of Drugs and Diseases, Vol 3, No 1 (2014), Pagination: 294-297Abstract
Objective: The present study was carried out for investigating the antibacterial activity of sliver nanoparticles (Ag NPs) biologically synthesized from the medicinal plant Aegle marmelos.
Methods: The silver nanoparticles from Aegle marmelos was impregnated onto a blank disks with different concentrations viz., 1mM, 3 mM and 5 mM and tested for its antimicrobial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), Pseudomonas aeuruginosa (ATCC 27853), and Candida albicans (ATCC 10231).
Results: The results indicated highest antimicrobial activity against E.coli and P. aeruginosa as a solution for finding.
Conclusion: The ability of Aegle marmelos silver nanoparticle to inhibit bacterial and fungi is an indication of its broad spectrum antimicrobial activity which could be a potential source of antibiotic which is cost effective and eco-friendly against drug resistant organisms.
Keywords
E. Coli , C. Albicans, P. Aeuruginosa , S. Aureus, Aegle Marmelos , Silver Nanoparticles- Antimicrobial Activity of Silver Nanoparticles from Swietenia Mahagoni
Authors
1 Department of Medical Biochemistry, University of Madras, Chennai-600113, IN
2 Department of Biochemistry JBAS College, Chennai 6000 018, IN
3 CLRI, Chennai 600020, IN
4 PNG University of Technology, PG
5 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai-600005, IN
Source
Indian Journal of Medicine and Healthcare, Vol 3, No 1 (2014), Pagination: 310-313Abstract
Objective: To produce natural, non-toxic biologically synthesized silver nanoparticles (Ag NPs) with antimicrobial activity from plant source, Swietenia mahagoni as a remedy for multi-resistant drug microbes.
Methods: The antimicrobial activity of these Ag NPs from Swietenia mahagoni was tested against three bacterial species, E.coli, S. aureus, P. aeruginosa and a fungal species, C. albicans.
Results: The experimental results exhibited satisfactory inhibitions against all tested microorganisms when used at different concentrations viz., 1mM, 3 mM and 5 mM; 3 mM (20 mg/500 μl distilled water) offered the highest sensitivity for E.coli and P. aeruginosa.
Conclusion: Green-synthesized antibacterial agent from Swietenia mahagoni showed good antibacterial and antifungal activity.
Keywords
E. Coli, C. Albicans , P. Aeuruginosa, Pithecellobium Dulce, S. Aureus- Comparison of Antimicrobial Activities of Silver Nanoparticles Synthesized from Dysoxylum parasiticum
Authors
1 The Papua New Guinea University of Technology, Lae, Morobe Province, Private Mail Bag., PG
2 University of Madras, Taramani Campus, Chennai-600113, IN
3 JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Medicine and Healthcare, Vol 4, No 2 (2015), Pagination: 1-5Abstract
Background/Objectives: In current trend biological synthesis of metallic nanoparticles is gaining importance because it is reliable and eco-friendly.
Methods/Statistical analysis: The present work leads to the synthesis of nanoparticles from 3mM and 5mM AgNO3 solution through aqueous leaf extract of Dysoxylum parasiticum as reducing as well as capping agent by employing boiling method and microwave irradiation method. Synthesized nanoparticles are characterized under UV-Vis spectroscopy at the range of 200-800nm.
Findings: Green synthesized silver nanoparticle showed zone of inhibition against tested microorganisms. The leaf extract shows higher antimicrobial activity found by disc diffusion method. Furthermore, determination of elemental silver was done by the Atomic Absorption Spectroscopy (AAS). The phytochemical constituent determination shows the positive results expect for the test for terpenoids and test for the reducing sugar.
Improvements/Application: Both Boiling Method and Microwave Irradiation Method are effective in synthesizing of silver nanoparticles using the leaves extracts of plants.
Keywords
Dysoxylum parasiticum, Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation and Boiling Method.- Comparison of Antimicrobial Activities of Silver Nanoparticles Synthesized from Sphaerostephanos asplenioides J. Sm
Authors
1 The Papua New Guinea University of Technology, Lae, Morobe Province, Private Mail Bag, PG
2 University of Madras, Taramani Campus, Chennai-600113, IN
3 JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Medicine and Healthcare, Vol 4, No 2 (2015), Pagination: 1-6Abstract
Background/Objectives: Green nanotechnology is generating attention of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, the possible role of Sphaerostephanos asplenioides J. Sm extract in reducing silver nitrate into silver nanoparticle is highlighted.
Methods/Statistical analysis: A comparative study was made in preparing the silver nanoparticles using the boiling method and microwave irradiation method for the S. asplenioides. The synthesis of silver nanoparticles were prepared by adding silver nitrate solution [3mM] and [5mM] to the plant extract. The silver nanoparticles were characterized using the UV-Visible Spectroscopy.
Findings: The antimicrobial assay was carried out using the disc diffusion method which showed promising antibacterial effects against the 15 tested microorganisms. The phytochemical constituent determination shows the positive results expect for the test for terpenoids and test for the reducing sugar.
Improvements/Application: It can be concluded that both methods (Boiling Method and Microwave Irradiation Method) are effective in synthesizing of silver nanoparticles using the leaves extracts of plants.
Keywords
Sphaerostephanos asplenioides J. Sm., Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation And Boiling Method.- Antimicrobial Activity of Synthesized Silver Nanoparticles and Phytochemical Screening of the Aqueous Extract of Antiaris toxicaria
Authors
1 Department of Applied Sciences, PNG University of Technology, LAE, PG
2 Deptartment of Medical Biochemistry, University of Madras,Taramani Campus, Chennai-600113, IN
3 Dept. of Biochemistry, JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Drugs and Diseases, Vol 4, No 1 (2015), Pagination: 1-4Abstract
Background/Objectives: Antiaris toxicaria is used as a traditional medicine around many parts of Papua New Guinea. The main aim of this study was to evaluate the compatibility of A. toxicaria by carrying out the phytochemical screening, synthesizing silver nanoparticles using silver nitrate and to determine their antimicrobial activity.
Methods/Statistical analysis: Antimicrobial activity was determined by agar paper disk diffusion essay and the zone of inhibition was measured against 14 microorganisms.
Results: All the test microorganisms were highly susceptible to the green synthesis of silver nanoparticles. Phytochemical screening of A. toxicaria reveals the presence of alkaloids, glycosides, terpenoids, reducing sugars, saponins and phenolic compounds.
Conclusion/Application: The nanoparticles synthesized from the aqueous plant extract of A. toxicaria contains the phytochemicals and it is proved to have the antimicrobial activity.
Keywords
Antiaris toxicaria, Antimicrobial Activities, Silver Nano Particles, Phytochemical Screening.- Comparison of Antimicrobial Activities of Silver Nanoparticles Synthesized from Bridelia minutiflora Hook. F. through Boiling Method and Microwave Irradiation Method
Authors
1 Dept of Applied Sciences, The Papua New Guinea University of Technology, Lae, Morobe Province, Private Mail Bag., PG
2 Dept of Medical Biochemistry, University of Madras, Taramani Campus, Chennai-600113, IN
3 Dept of Biochemistry, JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of Drugs and Diseases, Vol 4, No 1 (2015), Pagination: 1-5Abstract
Background/Objectives: The synthesis of nanoparticles from biological processes is evolving a new era of research interests in nanotechnology. In this study, the possible role of Bridelia minutiflora Hook. F. (B.minutiflora) extract in reducing silver nitrate into silver nanoparticle is highlighted.
Methods/Statistical analysis: The synthesis of silver nanoparticles were prepared by adding silver nitrate solution [3mM] and [5mM] to the plant extract. The silver nanoparticles were characterized using the UV-Visible Spectroscopy. The antimicrobial assay was carried out using the disc diffusion method.
Findings: A comparative study was made in preparing the silver nanoparticles using the boiling method and microwave irradiation method for the B. minutiflora. The results showed promising antibacterial effects against the 15 tested microorganisms. The phytochemical constituent determination shows positive test for terpenoids and reducing sugar.
Keywords
Bridelia minutiflora Hook F., Antimicrobial Activity, Silver Nanoparticles, Silver Nitrate, Microwave Irradiation and Boiling Method.- Green Synthesis and Characterisation of Silver Nanoparticles from the Medicinal Plant Pithecellobium Dulce
Authors
1 Dept of Biochemistry, Dept of Medical Biochemistry University of Madras, Chennai, IN
2 PNG University of Technology, PG
3 Department of Biochemistry, JBAS College, Chennai, IN
4 Tannary Department, CLRI, Adyar. Chennai, IN
5 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai-600005, IN
Source
Indian Journal of NanoScience, Vol 2, No 8 (2014), Pagination: 04-09Abstract
Objective: To introduce a cost effective and eco-friendly technique of preparing silver nanoparticles (Ag NP) from the medicinal plant, Pithecellobium dulce.
Methods: Ag NP of different concentration was prepared and characterized by using UV-Vis Spectrophotometer, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy, X-Ray Diffraction (XRD), and Fourier Transform Infra Red (FTIR).
Results: Studies by UV-Vis spectroscopy showed absorption spectra of silver nanoparticles at an absorbance peak at 475nm, in all the three different concentrations (1mM, 3mM and 5mM). The size of Ag NP ranged 50-70nm and appeared to be dominantly spherical (and occasionally triangular). The XRD pattern revealed that these silver nanoparticles have a crystalline nature.
Conclusion: These nanoparticles produced by green synthesis proved stable in solution and can have promising role in nano-medicine.
Keywords
Pithecellobium Dulce, Bioreduction, Silver Nanoparticles- Antimicrobial Activity of Synthesized Silver Nanoparticles and Phytochemical Screening of the Aqueous Extract of Mussaenda ferruginea
Authors
1 Department of Applied Sciences, PNG University of Technology, LAE, PG
2 Deptartment of Medical Biochemistry, University of Madras, Taramani Campus, Chennai600113, Chennai, IN
3 Dept of Biochemistry, JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of NanoScience, Vol 3, No 2 (2015), Pagination: 1-4Abstract
Background/Objectives: Mussaenda ferruginea is used as a traditional medicine around many parts of Papua New Guinea. It is used traditionally in the treatment of malaria and fever. The main aim of this study was to evaluate the compatibility of M. ferruginea in synthesizing silver nanoparticles and determine their antimicrobial activity.
Methods/Statistical analysis: 3mM and 6mM concentration of silver nanoparticles were prepared and checked for the antimicrobial activity using agar paper disc diffusion assay and measuring the zone of inhibition against 14 microorganisms. Also the photochemical screening was carried out for the aqueous extract of the plant.
Findings: All the microorganisms used were found to be highly susceptible to the synthesized silver nanoparticles. Phytochemical screening of M. ferruginea reveals the presence of alkaloids, glycosides, terpenoids, reducing sugars, saponins and phenolic compounds.
Improvements/Application: All the microorganisms are susceptible to silver nanoparticles but are resistant to the plant extract.
Keywords
Mussaenda ferruginea, Antimicrobial Activities, Agar well Diffusion Assay, Phytochemical Screening.- Antimicrobial Activity of Synthesized Silver Nanoparticles and Phytochemical Screening of the Aqueous Extract of Similax latifolia
Authors
1 Dept of Applied Sciences, PNG University of Technology, LAE, PG
2 Dept of Medical Biochemistry, University of Madras,Taramani Campus, Chennai 600113, IN
3 Dept of Biochemistry, JBAS College for Women, Teynampet, Chennai-600018, IN
Source
Indian Journal of NanoScience, Vol 3, No 2 (2015), Pagination: 1-4Abstract
Background/Objectives: Similax latifolia is used as a traditional medicine around many parts of Papua New Guinea. The main aim of this study was to evaluate the compatibility of S. latifolia in synthesizing silver nanoparticles and determine their antimicrobial activity.
Methods/Statistical analysis: Antimicrobial activity was carried out via Agar Paper Disk diffusion essay and measuring the zone of inhibition against 14 microorganisms.
Findings: All the microorganisms used were found to be highly susceptible to the synthesized silver nanoparticles. Phytochemical screening of S. latifolia reveals the presence of alkaloids, glycosides, terpenoids, reducing sugars, saponins and phenolic compounds.
Improvements/Application: The nanoparticle synthesized from the aqueous plant extract of S. latifolia proved to have the antimicrobial activity.